A 58 year old phakic female presented with blurred vision in the right eye. BCVA improved to 20/30 at 2 weeks and 20/25 at 9 months.Ĭase 5. At 2 weeks, SD-OCT showed release of the VMA with central foveal thickness of 326 um (Fig. The patient was treated with an intravitreal injection of 0.3 cc 100% SF6 and concurrent anterior chamber paracentesis. SD-OCT of the macula confirmed the ERM with VMA (Fig. Retinal examination revealed an ERM and RPE mottling. A 76 year old phakic female with mild non-proliferative diabetic retinopathy presented with blurred vision in the right eye. At 1 month, SD-OCT revealed resolution of the VMA and central foveal thickness of 252 um (Fig. SD-OCT of the macula confirmed VMA with central foveal thickness of 277 um (Fig. Retinal examination showed mild thickening and RPE mottling in the macula. A 76 year old phakic female with low risk ARMD presented with blurred vision in the left eye. Central foveal thickness improved to 252 um and BCVA acuity improved to 20/100.Ĭase 3. At 1 month, SD-OCT revealed partial release of the VMA with development of a lamellar defect (Fig. SD-OCT of the macula confirmed VMA with a stage 1b macular hole (Fig. Retinal examination showed the clinical appearance of a stage 1b hole. An 84 year old pseudophakic female with diabetes mellitus without retinopathy and low risk age related macular degeneration (ARMD) presented with blurred vision in the right eye. Pre-operative SD-OCT images of patients 1 through 6 (A-F) and SD-OCT images following pneumatic vitreolysis in patients 1 through 6 (a-f).Ĭase 2. Best corrected visual acuity remained stable at 20/60. A lamellar macular defect was also noted on SD-OCT. At 1 week, the VMA had released and CFT on SD-OCT had improved to 253 um Fig. The patient was treated with an intravitreal injection of 0.3 cc 100% sulfur hexafluoride (SF6) and concurrent anterior chamber paracentesis. 1A) and no evidence of epiretinal membrane (ERM). Spectral domain optical coherence tomography (SD-OCT) imaging of the macula confirmed vitreomacular adhesion (VMA) with a central foveal thickness of 391 um (Fig. Retinal examination showed mottling of the retinal pigmented epithelium (RPE) in the left eye. Best-corrected visual acuity (BCVA) at presentation was 20/60 in the left eye. A 78 year old phakic female with diabetes without retinopathy presented with blurred vision and distortion in the left eye.
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Just to be clear this down here is not a rectangle notice Rectangle you are dealing with the rectangle and so SO if all four corners are square or they're all come in at a right angle like this then you're dealing with a Sometimes you could call it a right angle so and notice I could put little small squares Shape right over here what you might see is the corners theĬorners all come in, sometimes is called square It's a quadrilateral Let's think about each of these so this One two three four sides as long as as four sides Looks like this, could even have a quadrilateral that look like this. These are all different special cases of quadrilaterals and actually you could even have a quadrilateral that Sides is quadrilateral Quad dri quadrilateral quadrilateral and when you see this quadĪt the beginning of the word it's a pretty good sign that you'reĭealing with the number four four sides, four sides that's what quadrilateral means but Well if you count them you see that this one up here in purple has one two three four sides this one has one two three four sides, this has one two three four sides, this has one two three four sides. Over here these shapes are interesting how many This is a triangle has three sides note about these shapes Hear that's a good sign that you're dealing with the number Triangle Tri angle whenever you see this Tri over One two three if you have three sides we call that a Now whatĪbout the shape over here that has three sides one two three you could also say it has three corners This one right over here is not a circle, this one is. Shorter than this distance right over here so In that direction is going to be much shorter or just Oval is not a circle because if you have its center see right over there this distance going Like this to be what you might call this an oval. Length so that's the same length as is this as this is on his way to theĬenter the circle as that now if that the circle what would something Of the circle to the other side circle through the center that's always going to be the same Think about it is if we take the center the circle and if we go from one side Now what makes it a circle when you getįurther in math we come up with fancier ways to define a circle but one with Recognize it is the shape of the moon in the sky or atleast on a full moon and we call it a circle. I have a bunch of shapes here, let's thinkĪbout what they are so this first shape here, you probably B cell receptors can further diversify through somatic hypermutations during affinity maturation. V(D)J recombination selects two or three germline segments (Variable-V and Joining-J loci for TCR α and BCR light chains and the V, Diversity-D, and J loci for TCR β and BCR heavy chains) from a library of germline genes, and assembles them while deleting base pairs and inserting other non-templated ones at the junctions (Fig. Probabilistic assignment of recombination scenarios Applied to BCRs, IGoR learns a context-dependent hypermutation model to identify hotspots, which allows for a comprehensive analysis of the mutational landscape of BCRs. IGoR used as a sequence generator produces an arbitrary number of randomly rearranged sequences with the same statistics as in the data set. IGoR’s performance at identifying the correct VDJ recombination scenario is two times better than current state-of-the-art methods. Using these statistics, for each sequence IGoR outputs a whole list of potential recombination and hypermutation scenarios, with their corresponding likelihoods. We present a flexible computational method and software tool, IGoR (Inference and Generation of Repertoires), that processes raw immune sequence reads from any source (cDNA or gDNA) and learns unbiased statistics of V(D)J recombination and somatic hypermutations. Quantitatively characterizing the diversity and the biases of these mechanisms remains a challenge for understanding adaptive immunity and applying RepSeq for diagnostics. Standard assignments introduce systematic errors when describing this inherently stochastic process. Different germline segments can recombine with each other with different frequencies, and the number of insertions and deletions is random, so that the overall receptor generation process cannot be described deterministically. However, each receptor sequence can be generated in a large number of ways, which we call “scenarios,” comprising the processes leading to pre-selection receptors: recombination of germline segments, insertions and deletions, and hypermutations. A growing number of algorithms and software tools have been designed to address the new challenges of RepSeq, in particular sequence analysis, germline assignment and clone construction 5, 6, 7, 8, 9, 10. The recent advent of high-throughput immune repertoire sequencing (RepSeq) 1, 2, 3, 4 gives us direct insight into the diversity of B cell and T cell receptor (BCR and TCR) repertoires with great potential to change the way we diagnose, treat, and prevent immune system-related disorders. The adaptive immune system recognizes pathogens by binding their antigens to specific surface receptors expressed on T and B cells. Tribal Pay allows them to make payments to merchants and suppliers that don’t accept credit cards. 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